Publications

1998-present (while at UAH)

• González-Ramírez, L.A., Moreno A., Ng, J.D. and García-Ruiz, J.M. (2022). Investigations on the Role of Iron (III) and Silica-Iron (III) for DNA Protection Against Highly Intense UV Radiation: Tracking the Connection of Prebiotic Chemistry to Biology. Astrobiology. In press. (Ahead of print access: https://doi.org/10.1089/ast.2022.0004)
• Petroff, A., Weir, R.L, Yates, C.R., Ng, J.D. and Baudry, J. (2021).  Sequential Dynamics of Stearoyl-CoA Desaturase-1(SCD1)/Ligand Binding and Unbinding Mechanism: A Computational Study.  Biomolecules 11:1435
• Havlickova, P., Brinsa, V., Brynda, J., Pachl, P., Prudnikova, T., Mesters, J.R., Kascakova, B., Kuty, M., Pusey, M.L., Ng, J.D., Rezacova, P. and Smatanova, I.K.(2019).  A novel structurally characterized haloacid dehalogenase superfamily phosphatase from Thermococcus thioreducens with diverse substrate specificity.  Acta Cryst. D75, 743-752. 
• Pusey, M., Barcena, J., Morris, M., Singhal, A., Yuan, Q. and Ng, J.D. (2015). Trace fluorescent labeling for protein crystallization.  Acta Crystallogr Sect F Struct Biol Cryst Commun, 71, 806-814.
• Ng, J. D., Baird, J. K., Coates, L., García-Ruiz, J.M., Hodge, T.A. and Huang, S. (2015).  Large volume protein crystal growth for Neutron Macromolecular Crystallography. Acta Crystallogr Sect F Struct Biol Cryst Commun, 71, 358-370.
• Coates, L., Tomanicek, S., Shrader, T., Weiss, K., Ng, J.D., Juttner, P. and Ostermann, A. (2014). Cryogenic neutron protein crystallography: routine methods and potential benefits. Journal of Applied Crystallography. 47:1431-1434.
• Ogungbe, I.V., Ng, J.D. and Setzer, W.N. (2013).  Interactions of antiparasitic alkaloids with Leishmania protein targets: a molecular docking analysis. Future Med Chem. 15:1777-99.
• Ng, J.D., Dowell, J.J., Kar, A.K., Hansen, K., Thundat, T. and George, M.A. (2013). Measurement of temperature induced unfolding of DNA hairpins by microcantilever sensors.  Open Journal of Applied Biosensor. 2:78-82.
• Tomanicek, S.J., Standaert, R.F., Weiss, K.L., Ostermann, A., Schrader, T.E., Ng, J.D.  Coates L. (2013).  Neutron and X-ray crystal structures of a perdeuterated enzyme inhibitor complex reveal the catalytic proton network of the Toho-1 β-lactamase for the acylation reaction. J. Biol. Chem. 288(7):4715-4722.
• Hughes, R.C., Coates, L., Blakeley, M.P., Tomanicek, S.J., Langan, P., Kovalesvsky, A., García-Ruiz, J.M. and Ng, J.D. (2012).  Inorganic pyrophophatase crystals from Thermococcus thioreducens for X-ray and neutron diffraction.  Acta Crystallogr Sect F Struct Biol Cryst Commun 68:1482-1487.

• Chu, X.Q., Gajapathy, M., Weis, K., Mamontov, E., Ng, J.D. and Coates, L. (2012).  Dynamic Behavior of Oligomeric Inorganic Pyrophosphatase Explored by Quasielastic Neutron Scattering. The Journal of Physical Chemistry 116:9917-9921.

• Kuznetsov, Y.G., Dowell, J.J., Gavira, J.A., Ng, J.D. and McPherson, A. (2010). Biophysical and atomic force microscopy characterization of the RNA from satellite tobacco mosaic virus. Nucleic Acids Research 38(22):8284-94.
• Tomanicek, S.J., Hughes, R.C., Ng, J.D. and Coates, L. (2010). Structure of the endonuclease IV homologue from Thermotoga maritima in the presence of active-site divalent metal ions. Acta Crystallogr Sect F Struct Biol Cryst Commun 66:1003-1012.
• Kantardjieff, K.A., Lind, C. Ng, J.D. and Santarsiero, B.(2010).  Efforts to enhance coverage of crystallography in United States secondary education. J. Applied Crystallography 43, 1181–1188.

• Otalora, F., Gavira, J.A., Ng, J.D., García-Ruiz, J.M. (2010) Counter-Diffusion Methods Applied to Protein Crystallization. Progress in Biophysics and Molecular Biology 101:26-37. 
• Hughes, R.C., Tomanicek, S.J., Ng, J.D. and Coates, L. (2009). Purification, crystallization and preliminary crystallographic analysis of a thermostable endonuclease IV from Thermotoga maritima. Acta Crystallogr Sect F Struct Biol Cryst Commun.65:1317-1319.
• Byrne-Steele, M., Hughes, R.C. and Ng, J.D. (2009). Recombinant production, crystallization and preliminary X-ray analysis of PCNA from the psychrophilic archaeon Methanococcoides burtonii DSM 6242. Acta Crystallogr F65 :1131-1135.
• Byrne-Steele, M. and Ng, J.D. (2009). Expression, purification and preliminary X-ray analysis of proliferating cell nuclear antigen from the archaeon Thermococcus thioreducens. Acta Crystallogr F65 :906-909.
• Wilson, R.C., Hughes, R.C., Flatt, J.W., Meehan, E.J., Ng, J.D. and Twigg, P.D. (2009). Structure of full-length ubiquitin-conjugating enzyme E2-25 K(Huntingtin-interacting protein 2).  Acta Crystallogr Sect F Struct Biol Cryst Commun. F65:440-444.
• Marsic, D., Flaman, J.M. and Ng, J.D. (2008).  New DNA polymerase from the hyperthermophilic marine archaeon Thermococcus thiroreducens.   Extremophiles 12:775-788.
• Marsic, D., Hughes, R.C., Byrne-Steele, M.L. and Ng, J.D. (2008).  PCR-based gene synthesis to produce recombinant proteins for crystallization. BMC Biotechnology. 8:44.
• Ng, J.D., Clark P., Stevens, R.C. and Kuhn P. (2008). In situ X-ray analysis of proteins crystals in low birefringent and X-ray transmissive plastic micro-channels.  Acta Crystallogr D Biol Crystallogr D64:189-197.
• Shaw, N., Tempel W, Chang, J., Yang, H., Cheng, C., Ng, J.D., Rose, J., Rao, Z. Wang, B.C. Liu, Z.J, (2007). Crystal structure solution of a ParB-like nuclease at atomic resolution. Proteins 70:263-267.
• Ng, J.D., Stevens, R.C. and Kuhn P. (2008).  Protein crystallization in restricted geometry: advancing old ideas for modern times in structural proteomics.  Methods in Molecular Biology 426:363-376.
• Wilson, R.C., Hughes, R.C., Curto, E.V., Ng, J.D. and Twigg, P.D. (2007). Backbone ¹H, ¹⁵N, and ¹³C Resonance Assignments and Secondary Structure of a Novel Protein OGL-20P^T-358 from Hyperthermophile Thermococcus thioreducens  sp. nov.Molecules and Cells 24:437-440.
• Shaw, N. Cheng, C., Tempel, W. Chang, J. Ng, J.D., Wang, XY, Perrett, S., Rose, J., Rao, Z., Wang, B.C. and Liu, ZJ.  (NZ)CH…O contacts assist crystallization of a ParB-like nuclease.  BMC Struct. Biol. 7:46.
• Pikuta, E., Marsic, D., Itoh, T., Bej, A.K., Tang, J., Whitman, W., Ng, J.D., Garriott, O.K. and Hoover, R.B. (2007). Thermococcus thioreducens sp. nov., a novel hyperthermophilic, obligately sulfur-reducing archaeon from a deep-sea hydrothermal vent. Int. J Syst Evol Microbiol. 57:1612-1618.
• Baird, J.K., Caraballo, K. and Ng, J.D. (2007).  Kinetics of protein crystallization. Book Chapter in Focus on Crystal Growth Research (Ed: G.V. Karas) pp 171-192.
• Hughes, R.C. and Ng, J.D. (2007).  Can small laboratories do structural genomics?  Crystal Growth and Design 7:2226-2238.
• Pradhan, D., Marsic, D., Garriott, O., Meehan, E., and Ng, J.D. (2006). Isolation of Novel Alkaliphilic Bacteria from Lake Makat, Tanzania and Recombinant Expression of Two New Proteases. Proceedings of International Society of Extremophile and their Application (ISEA, Tokyo, Japan)3:330.

• Caraballo, K.G., Baird, J.K. and Ng, J.D. (2006). Kinetics of Supersaturation Decay in the Crystallization of Canavalin. Crystal Growth and Design. 6:874-880.
• Ng, J.D. and García-Ruiz, J.M. (2006).  Counter-diffusion capillary crystallization for structural genomics. Trends in Drug Discovery 3:36.
• García-Ruiz, J.M. and  Ng, J.D. (2006). Counter-diffusion capillary crystallization for high throughput applications. In Protein crystallization strategies for structural genomics (N. E. Chayen, ed) International University Line Chapter 5.
• Pusey, M.L., Liu, Z-J, Tempel, W., Praissman, J., Lin, D., Wang, B-C., Gavira, J.A. and Ng, J.D. (2005).  Life in the fast lane for protein crystallization and X-ray crystallography.  Progress in Biophysics and Molecular Biology 88:359-386.
• Liu, Z. J.; Tempel, W.; Ng, J. D.; Lin, D.; Shah, A. K.; Chen, L.; Horanyi, P. S.; Habel, J.; Kataeva, I.; Xu, H.; Yang, H.; Chang, J. C.; Huang, L.; Chang, S.; Zhou, W.; Lee, D.; Praissman, J.; Zhang, H.; Newton, M. G.; Rose, J. P.; Richardson, J. S.; Richardson, D. C.; Wang, B.-C.(2005).  The High Throughput Protein to Structure Pipeline at SECSG. Acta Crystallogr D Biol Crystallogr 61:679-84.
• Bernhardsdotter, E.C.M.J., Ng, J.D., Garriott, O.K. and Pusey, M.L. (2005).  Enzymatic properties of an alkaline chelator resistant a-amylase form an alkaliphilic Bacillus sp. Isolate L1711. Process Biochemistry 40:2401-2408.
• Liu, Z. J.; Shah, A. K.; Habel, J.; Ng, J. D.; Kataeva, I.; Xu, H.; Horanyi, P.; Yang, H.; Chang, J.; Huang, L.; Chang, S.; Tempel, W.; Chen, L.; Zhou, W.; Lee, D.; Lin, D.; Zhang, H.; Newton, G.; Rose, J.; Wang, B.-C.(2005). Salvaging Pyrococcus furiosus protein targets at SECSG. J Struct Funct Genomics 6:121-127.
• Wang, B. C.; Adams, M.; Dailey, H.; DeLucas, L.; Luo, M.; Rose, J.; Bunzell, R.; Dailey, T.; Habel, J.; Horanyi, P. S.; Jenney, F.; Karaveg, K.; Lee, H.-S.; Li, S.; Li, S. C.; T.; Lin, D.; Liu, Z. J.; Luan, C.-H.; Mayer, M.; Nagy, L.; Newton, M. G.; Ng, J. D.; Poole, F.; Shah, A. K.; Sugar, F. F.; Xu, H. (2005). Protein production and crystallization at SECSG – An overview. Journal of Structural and Functional Genomics. 6:233-43.
• Ng, J.D. (2004).  DNA to protein and perspectives on protecting the fruits of structural genomic research.  Journal of International Law 1: 40-42.
• Ng, J.D., Gavira, J.A. and García-Ruiz, J.M. (2003).  Protein crystallization by capillary counter-diffusion for applied crystallographic structure determination.  Journal of Structural Biology 142:218-231.
• Barba-de la Rosa, A. P., Ng, J., Day, J. and McPherson, A. (2003) Structural characterization of satellite tobacco mosaic virus RNA. Agrocienda 37, 503-510.
• Ng, J.D.  (2002).  Space grown crystals are more useful for structure determination. Annals of the New York Academy of Sciences 974:598-609.
• Lorber, B., Theobald-Dietrich, A., Charron, C., Sauter, C. Ng, J.D., Zhu, D.W. and Giege, R. (2002).  From conventional crystallization to better crystals from space: a review on pilot crystallogenesis studies with aspartyl-tRNA synthetases. Acta Crystallographica D58: 1674-1680
• Gavira, J.A., Lopéz-Jaramillo, J., Toh, D., García-Ruiz, J.M. and Ng, J.D.  (2002). Ab initio crystallographic structure determination of insulin form protein to electron density without crystal handling. Acta Crystallographica Section D58 :1085-1254.
• Ng, J.D., Sauter, C., Lorber, B., Kirkland, N., Arnez, J. and Giegé, R. (2002).  Comparative analysis of space- and earth-grown crystals of an aminoacy-tRNA synthetase: Space-grown crystals are more useful for structural determination.  Acta Crystallographica Section D58:645-652.
• Zhu, D.W., Lorber, B., Sauter, C., Ng, J.D., Benas, P., Le Grimellec, C. and Giege, R. (2001). Growth kinetics, diffraction properties, and effect of agarose on the stability of a novel crystal form of T. thermophilus aspartyl-tRNA synthetase-1 Acta Crystallographica Section D. Acta Cryst. D57, 552-558.
• Charron, C., Sauter, C., Zhu, D.W., Ng, J.D. Kern, Lorber,B. and Giegé, R. (2001). Packing contacts in orthorhombic and monoclinic crystals of a thermophilic aspartyl-tRNA synthetase favor the hydrophobic regions of the protein. J. Cryst. Growth 232: 376-386.
• Marsic, D., Pikuta, E.V., Hoover, R. and Ng, J.D. (2001). Cloning of the 16S ribosomal RNA gene of a psychrophilic bacterium from the Alaskan Fox Permafrost Tunnel. Proceedings to the International Society for Optical Engineering: Instruments, Methods and Missions for Astrobiology II .3755:163-173.
• Roberts, P.H., Zhou, X., Holmes, A., Ranson, H., Small, G., Hemingway, J., Ng, J.D., Chen, L. and Meehan, E.J. (2001). Multi-form crystallization of agGST1-6, a recombinant glutathione S-transferase from a DDT-Resistant strain of Anopheles gambiae. Acta Crystallographica Section D57:134-136.
• Green, M.E., Kirkland, N. and Ng, J.D. (2001). Effect of a mutation at arginine 301 on the stability, crystal quality and the preliminary crystallographic analysis of recombinant canavalin from Canavalia ensiformis. J. Cryst. Growth 232:387-398.
• Hoover, R.B., Pikuta, E. V., Marsic, D. and Ng, J.D. (2001). Anaerobic Psychrophiles from Alaska, Antarctica, and Patagonia: Implications to Possible Life on Mars and Europa. Proceedings to the International Society for Optical Engineering: Instruments, Methods and Missions for Astrobiology II . 4495:313.
• Marsic, D., Hoover, R.B., Gilinchinsky, D.A. and Ng, J.D. (2000). Gene cloning of the 18S rRNA for ancient viable moss from the perma frost of northeastern Siberia. Proceedings to the International Society for Optical Engineering: Instruments, Methods and Missions for Astrobiology II . 3755:163-173.

• Lorber, B., Ng, J.D., Lautenschlager, P., and Giegé (2000) Growth kinetics and motion of thaumatin crystals during USML-2 and LMS microgravity missions and comparisons with earth controls. J. Crystal Growth 208:665-677.
• Lorber, B., Sauter, C., Ng, J.D., Zhu, D.W., Giege, R., Vidal, O., Robert, M.C. and Capelle. (1999). Characterization of protein and virus crystals by quasi-planar wave X-ray topography: a comparison between crystals grown in solution and in agarose. J. Cryst. Growth 204:357-368.
• Sauter, C., Ng, J., Lorber, B., Keith, G., Brion, P., Hosseini, M.W., Lehn, J-M and Giegé, R. (1999). Additives for the crystallization of proteins and nucleic acids. J. Cryst. Growth 196:365-376.

Other publications prior to UAH are within the complete list of published work in MyBibliography:

https://www.ncbi.nlm.nih.gov/myncbi/1TSF_2nel55kf/bibliography/public/